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: Check with your university's core facility or IT department. Many institutions purchase bulk licenses that allow students and staff to use FlowJo at a fraction of the cost or completely free. apply gating strategies

For researchers willing to learn a bit of code, the R ecosystem is the gold standard for robust, reproducible cytometry analysis. The flowCore and flowWorkspace libraries available through Bioconductor allow you to import FCS files, apply gating strategies, and perform advanced statistical modeling.